GRADUATE SCHOOL OF HEALTH SCIENCES
histoloji_ve_embriyoloji_(vet.) Yüksek Lisans - Doktora - Tessiz Yüksek Lisans
Course Name   Cell isolation and identification techniques
Semester Course Code Theoretical / Practice time ECTS
1 3242000010 0 / 2 1
Course Degree Master's Degree
Course Language Turkish
Format of Delivery: Face to Face
Course Coordinator Prof. Dr.Emrah Sur
Coordinator e-mail emrahsur selcuk.edu.tr
Instructors
Prof. Dr.Emrah Sur
Asistant Instructors
Course Objectives Teaching the cell isolation and identification techniques.
Basic Sciences Engineering Scinces Social Sciences Educational Sciences Artistic sciences Medical Science Agricultural sciences
0 0 0 0 0 100 0
Course Learning Methods and Techniquies
Practice
Week Course Content Resource
1 Harvesting peritoneal cells Basic Histology (Junqueira, LC and Carneiro, J, 10th Edition, 2003)
2 Liver lymphocyte harvesting Basic Histology (Junqueira, LC and Carneiro, J, 10th Edition, 2003)
3 Osteoblast harvesting Basic Histology (Junqueira, LC and Carneiro, J, 10th Edition, 2003)
4 Preparation of low density collagenase-digested splenocytes Basic Histology (Junqueira, LC and Carneiro, J, 10th Edition, 2003)
5 Subculturing monolayer cell cultures Basic Histology (Junqueira, LC and Carneiro, J, 10th Edition, 2003)
6 Use of dissociating enzymes Basic Histology (Junqueira, LC and Carneiro, J, 10th Edition, 2003)
7 Collagenase Basic Histology (Junqueira, LC and Carneiro, J, 10th Edition, 2003)
8 Trypsine Basic Histology (Junqueira, LC and Carneiro, J, 10th Edition, 2003)
9 Elastase Basic Histology (Junqueira, LC and Carneiro, J, 10th Edition, 2003)
10 Hyaluronidase Basic Histology (Junqueira, LC and Carneiro, J, 10th Edition, 2003)
11 Papain Basic Histology (Junqueira, LC and Carneiro, J, 10th Edition, 2003)
12 Chymotrypsine Basic Histology (Junqueira, LC and Carneiro, J, 10th Edition, 2003)
13 Deoxyribonüclease I Basic Histology (Junqueira, LC and Carneiro, J, 10th Edition, 2003)
14 Trypsine inhibitor Basic Histology (Junqueira, LC and Carneiro, J, 10th Edition, 2003)
15 FINAL EXAM
Assesment Criteria   Mid-term exam Final exam
  Quantity Percentage Quantity Percentage  
Term Studies : - -
Attendance / Participation : - -
Practical Exam : - -
Special Course Exam : - -
Quiz : - -
Homework : 1 10
Presentations and Seminars : 1 10
Projects : - -
Workshop / Laboratory Applications : 1 10
Case studies : - -
Field Studies : - -
Clinical Studies : - -
Other Studies : - -
Mid-term exam   - -
Final exam   1 70
ECTS WORK LOAD TABLE   Number Duration
Course Duration : 14 2
Classroom Work Time : 0 0
Presentations and Seminars : 1 1
Course Internship : - -
Workshop / Laboratory Applications : 0 0
Field Studies : - -
Case studies : - -
Projects : - -
Homework : 0 0
Quiz : 0 0
Mid-term exam : - -
Final Exam : 1 1
ECTS 1
No COURSE LEARNING OUTCOMES CONTRIBUTION
D.Ö.Ç. 1 Students learn the functions of collagenase, papain, elastase and hyaluronidase, 4
D.Ö.Ç. 2 learn to use trypsin inhibitors, 4
D.Ö.Ç. 3 learn harvesting methods of the peritoneal cells, lympocytes, and osteoblasts, 4
D.Ö.Ç. 4 recognize the different cell types, 4
D.Ö.Ç. 5 learn to identification of cells from body fluids such as urine, saliva, semen, swabs from the female and male genital tract, 4
D.Ö.Ç. 6 learn the concept of the standard density gradient, 4
D.Ö.Ç. 7 explain the cell viability determination, 4
D.Ö.Ç. 8 apply the appropriate methods of the cenyrifugation, 4
D.Ö.Ç. 9 understand of the tissue fractionation, 4
D.Ö.Ç. 10 learn the isolation medium, 4
D.Ö.Ç. 11 comment the relationship between the cells and some related diseases 4
* 1: Zayıf - 2: Orta - 3: İyi - 4: Çok İyi
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